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Cloning and mapping of telomere-associated sequences from Hordeum vulgare L.

✍ Scribed by Kilian, Andrzej ;Kleinhofs, Andris

Publisher
Springer
Year
1992
Tongue
English
Weight
586 KB
Volume
235
Category
Article
ISSN
0026-8925

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✦ Synopsis

We present a novel approach to the efficient cloning of telomere-associated sequences and demonstrate its application to the cloning and mapping of these sequences from barley. The method is a modification of the Vectorette PCR technique and allows specific amplification and cloning of subtelomeric sequences. Telomere-associated sequences isolated from barley include hypervariable, repetitive sequences. Polymorphisms detected by subtelomeric markers behaved as Mendelian factors and were mapped to the most distal positions of barley chromosomes 1, 2, and 4.

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