Isolation and Identification of Rare and Differentially Expressed Genes Using Subtractive Hybridization
β Scribed by R. Zebrowski; J. Thorman; T. Norred; R.E. Hurst
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 296 KB
- Volume
- 222
- Category
- Article
- ISSN
- 0003-2697
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FIGURE 1 -Expression of presumed differentially expressed cDNA fragments in neo6/melanoma hybrids by Northern blot and RT-PCR. Northern blot and RT-PCR analysis were done as described in Material and Methods.
Competitive hybridization is a simple yet powerful method that was developed to screen cDNA libraries for differentially regulated genes. The method is based on competition between unlabeled cDNA from the mRNA of one sample and labeled cDNA from another sample. By manipulating the amount of competin