✦ LIBER ✦

Competitive Hybridization: Theory and Application in Isolation and Quantification of Differentially Regulated Genes

✍ Scribed by Guang Yan Zhong; Joseph Riov; Raphael Goren; Doron Holland

Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 109 KB
Volume: 282
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.2000.4581

No coin nor oath required. For personal study only.

✦ Synopsis

Competitive hybridization is a simple yet powerful method that was developed to screen cDNA libraries for differentially regulated genes. The method is based on competition between unlabeled cDNA from the mRNA of one sample and labeled cDNA from another sample. By manipulating the amount of competing unlabeled cDNA, background signals from the nonregulated genes can be increased or reduced, enabling the signals from differentially regulated genes to be contrasted and to be identified in a quantitative manner. To demonstrate the feasibility of the method, we screened a citrus cDNA library for ethylene-induced genes and identified three genes with different levels of ethylene induction. The mathematical basis of the method and its possible application in gene chip technology are discussed.

📜 SIMILAR VOLUMES

Isolation and Identification of Rare and

Isolation and Identification of Rare and Differentially Expressed Genes Using Subtractive Hybridization

✍ R. Zebrowski; J. Thorman; T. Norred; R.E. Hurst 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 296 KB

Isolation of genes differentially expres

Isolation of genes differentially expressed in human primary myoblasts and embryonal rhabdomyosarcoma

✍ Michele Genini; Petra Schwalbe; Florence A. Scholl; Beat W. Schäfer 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 842 KB

Using a subtractive hybridization method, we have cloned 48 cDNAs which are expressed in human primary myoblasts but down-regulated in the embryonal-rhabdomyosarcoma (RMS) cell line RD. Twenty-nine sequences could be identified as coding for previously known gene products, while 19 encode unknown pr

Expression of housekeeping and immunopro

Expression of housekeeping and immunoproteasome subunit genes is differentially regulated in positively and negatively selecting thymic stroma subsets

✍ Ahmed Nil; Elke Firat; Vera Sobek; Klaus Eichmann; Gabriele Niedermann 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB

Identification of tissue-specific genes

Identification of tissue-specific genes in nasopharyngeal epithelial tissue and differentially expressed genes in nasopharyngeal carcinoma by suppression subtractive hybridization and cDNA microarray

✍ Bicheng Zhang; Xinmin Nie; Bingyi Xiao; Juanjuan Xiang; Shourong Shen; Jialei Go 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 251 KB 👁 1 views

## Abstract Suppression subtractive hybridization (SSH) was performed for isolation of tissue‐specific genes in nasopharyngeal epithelial tissue, by use of cDNAs from human adult nasopharyngeal epithelial tissue as tester and mixed cDNAs from esophagus, lung, liver, heart, stomach, spleen, skeletal

Detection of differentially expressed ge

Detection of differentially expressed genes in prostate cancer by combining suppression subtractive hybridization and cDNA library array

✍ Kati P. Porkka; Tapio Visakorpi 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB 👁 2 views

Product differentiation, competition and

Product differentiation, competition and regulation of new drugs: the case of statins in four European countries

✍ Panos Kanavos; Joan Costa Font; Alistair McGuire 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 175 KB

## Abstract This paper examines the determinants of competition in the statins market across four European countries over the 1991–2002 period. Using IMS data from the UK, Germany, France and the Netherlands, the paper analyses the potential existence of competition between branded statins prior to