Isolation and identification of mutants constitutive for aspartokinase III synthesis in Escherichia coli K 12
✍ Scribed by Boy, Emmanuelle; Borne, Françoise; Patte, Jean-Claude
- Book ID
- 120501630
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 632 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0300-9084
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A method was developed for the isolation of spontaneous mutants of Escherichia coli K-12 with deletions extending from the srl operon to the adjacent recA gene. The srl-recA deletion mutants were extremely sensitive to dNA-damaging agents; unable to support growth of the feckless red gam mutant lamb
Five mutants (called tnm) of Escherichia coli with impaired ability for transposition of Tn9 were isolated after treatment with ethyl methanesulfonate (EMS) or N-methyl-N'-nitro-N-nitrosoguanidine (NG). The map locations of the tnm mutations were determined by a combination of Hfr matings, F' episom