Isolation and identification of 3-deoxy-7-O-α-d-galactopyranosyl-d-manno-2-octulopyranosonate from the inner core region of the lipopolysaccharide of Escherichia coli EH100
✍ Scribed by Otto Holst; Eva Röhrscheidt-Andrzejewski; Hans-Peter Cordes; Helmut Brade
- Book ID
- 107725825
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 415 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0008-6215
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The dissaccharide D-galactosyl-3-deoxy-D-ma~o-2-octulosonic acid (Gal-KDO) from lipopolysaccharides (LPS) of the inner core region of the rough mutant EHl00 of E. coli 0100 was isolated after mild acid hydrolysis of LPS by means of dialysis, ion-exchange chromatography, gelfiltration and high-voltag
The title compound (PE-Kdo) was isolated after hydrolysis of the lipopolysaccharides of Escherichia coli K-12 strain W3100 and Salmonella minnesota strains R4 and R7, and the location of the 2-aminoethyl phosphate group at position 7 was established by 13C-n.m.r. spectroscopy. Derivatives of PE-Kdo