✦ LIBER ✦
Identification of D-galactose-(1 → 7)-3-deoxy-d-manno-2-octulo-pyranosonate as component of the inner core region of escherichia coli EH 100 lipopolysaccharide
✍ Scribed by O. Holst; E. Röhrscheidt-Andrzejewski; H.-P. Cordes; H. Brade
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 297 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0138-4988
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✦ Synopsis
The dissaccharide D-galactosyl-3-deoxy-D-ma~o-2-octulosonic acid (Gal-KDO) from lipopolysaccharides (LPS) of the inner core region of the rough mutant EHl00 of E. coli 0100 was isolated after mild acid hydrolysis of LPS by means of dialysis, ion-exchange chromatography, gelfiltration and high-voltage paper electrophoresis. By chemical analysis galactose and KDO were identified in a molar ratio of approximately 1 : 1. 13C-NMR and 'H-iWR studies, methylation analysis and GLC of the acetylated R-( -)-2-butyl-galactoside identified the structure