We isolated stable mutants from Trichosporon adeninovorans resistant to 2-deoxy-~-glucose. All mutants were altered in the C-catabolite repression and produced different amounts of glucoamylase and 8-glucosidase when grown on several carbon sources. In addition to the C-catabolite derepression of th
Isolation and characterization ofDaucus carotaL. cell lines resistant to 2-deoxy-D-glucose
β Scribed by John R. Stommel; Philipp W. Simon
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 441 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0167-6857
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Variants resistant to 2-deoxy-D-glucose have been isolated from a clonal line of pig kidney cells by serial cultivation in the presence of inhibitor. Hexokinase activity may be affected directly i n this system, since the oxidation of glucose to 6-phosphogluconate by extracts from sensitive and resi
The reaction of 2-amino-2-deoxy-D-glucose with lysine in water under simulated physiological conditions gave several browning products, with characteristic optical (lambda max 340 nm) and fluorescent properties (emission at 430 nm for excitation at 362 nm). The major product was isolated and charact