## Abstract Ubiquitin and ubiquitinβlike proteins are known to be covalently conjugated to a variety of cellular substrates via a threeβstep enzymatic pathway. These modifications lead to the degradation of substrates or change its functional status. The ubiquitinβactivating enzyme (E1) plays a key
Isolation and Characterization of Ubiquitin-activating Enzyme E1-domain Containing 1, UBE1DC1
β Scribed by Tonghai Dou; Shaohua Gu; Jianping Liu; Fei Chen; Li Zeng; Lingchen Guo; Yi Xie; Yumin Mao
- Publisher
- Springer
- Year
- 2005
- Tongue
- English
- Weight
- 513 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0301-4851
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The nucleotide sequence of the human cDNA encoding ubiquitin-activating enzyme E1 is more than 99% identical with the human A1S9T cDNA, a gene that has been shown to complement the temperature-sensitive mutant mouse cell line, tsA1S9. The amino acid sequences of the proteins encoded by these two cDN
## Abstract Protein ubiquitination plays critical roles in the regulation of multiple cellular processes including cell proliferation, signal transduction, oncogenesis, and hypoxic response. TS20 is a Balb3T3βderived cell line in which ubiquitination is inhibited by restrictive temperature. While T