✦ LIBER ✦

Isolation and characterization of thymidylate synthetase mutants ofXanthomonas maltophilia

✍ Scribed by Thomas P. West

Publisher: Springer
Year: 1989
Tongue: English
Weight: 315 KB
Volume: 151
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00413133

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✦ Synopsis

Thymidylate synthetase mutants of Xanthomonas maltophilia ATCC 13270 were isolated on a solid minimal medium containing 50 rag/1 thymidine and a high concentration of trimethoprim (500 rag/l). It was found that a high concentration of trimethoprim was required to prevent background growth of the wild-type strain. The isolated mutants could grow on thymidine or dTMP at a concentration of 50 mg/1 while they were unable to grow on 1000 mg/1 thymine or 50 mg/1 deoxyuridine. Thymidylate synthetase activity was assayed in the wild-type cells and in the mutant cells but only the wild-type cells contained measurable enzyme activity.

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