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Isolation and properties of yeast mutants affected in farnesyl diphosphate synthetase

โœ Scribed by C. Chambon; V. Ladeveze; A. Oulmouden; M. Servouse; F. Karst

Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
631 KB
Volume
18
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

Two yeast mutant strains auxotrophic for ergosterol and blocked in farnesyl diphosphate synthetase (EC 2.5.1.1) were isolated. Genetic analysis has shown that these mutant strains carry additional mutations in the ergosterol pathway besides erg20-1 and erg20-2 which affect FPP synthetase. The novel feature of these mutants is their ability to excrete prenyl alcohols (farnesol and geraniol). As geraniol is toxic for yeast cells, the above leaky mutations in FPP synthetase have to be associated with others in the sterol pathway, in order to slow down geraniol synthesis.

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