Isolation and characterization of three different carbohydrate chains from melanoma tissue plasminogen activator
β Scribed by Gunnar POHL; Lennart KENNE; Bo NILSSON; Monica EINARSSON
- Book ID
- 115125472
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 840 KB
- Volume
- 170
- Category
- Article
- ISSN
- 1432-1327
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Melanoma growth stimulatory activity (MGSA) is an acid and heat stable, auto-stimulatory growth factor which was first isolated from culture medium conditioned by the Hs294T human melanoma cell line. In this report, we describe the purification of MGSA from acid ethanol extracts of Hs294T tumors gro
Supernatants of a mclnnorna cell line were chromatographed on five affinity supports to ev;ilu;itr and compare purification protocols for isolation of tissue-type plasminogen activator (t-Pa). t-PA was adsorbed qunntitittively from cell culture supernatants by lysine-Sepharose, concanavulin -A-Sepha