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Isolation and characterization of ribosomal ribonucleic acid from the crustacean,Gecarcinus lateralis

✍ Scribed by Skinner, Dorothy M.

Publisher
John Wiley and Sons
Year
1968
Tongue
English
Weight
799 KB
Volume
169
Category
Article
ISSN
0022-104X

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✦ Synopsis

Methods for the isolation of ribosomal RNA (rRNA) from three crustacean tissues have been devised. These include precipitation of rRNA from total RNA preparations with 1M sodium chloride, to remove both melanin and ATP, or solubilization in buffer containing EDTA, to chelate Mg++ and Ca++ which, when present, form alcohol-insoluble complexes with ATP. By cosedimentation or gel electrophoresis with rRNA of known S ~O , ~, the sedimentation constants of crab rRNA have been determined to be 28 S and 18 S. The base compositions of the 28 S and 18 S rRNA's have been determined. Muscle, midgut gland and epidermis have been found to have higher concentrations of rRNA in the premolt period than in the intermolt period, with the maxima corresponding to the periods of maximum protein synthesis. Premolt epidermis has the highest rRNA content of the three tissues.

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