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Isolation and Characterization of Novel Peroxisome Biogenesis-Defective Chinese Hamster Ovary Cell Mutants Using Green Fluorescent Protein

✍ Scribed by Kamran Ghaedi; Atsushi Kawai; Kanji Okumoto; Shigehiko Tamura; Nobuyuki Shimozawa; Yasuyuki Suzuki; Naomi Kondo; Yukio Fujiki

Book ID: 115603233
Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 255 KB
Volume: 248
Category: Article
ISSN: 0014-4827
DOI: 10.1006/excr.1999.4413

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## Abstract A mutant (A204) of Chinese hamster ovary cells (CHO‐K1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3.3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact ce