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Isolation and characterization of mitochondria from goat hearts

โœ Scribed by Tester F. Ashavaid; Neena S. Kumbhat

Publisher
Association of Clinical Biochemistry of India
Year
2005
Tongue
English
Weight
853 KB
Volume
20
Category
Article
ISSN
0970-1915

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An improved method for isolation of mito
An improved method for isolation of mitochondria in high yields from normal, ischemic, and autolyzed rat hearts
โœ Jane A. Idell-Wenger; Lee W. Grotyohann; James R. Neely ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 603 KB

An improved procedure for the isolation of mitochondria in high yields from normal and oxygen-deficient myocardium is described. The heart muscle is digested with Nagarse and homogenized simultaneously using a Polytron tissue homogenizer. Mitochondria are isolated by differential centrifugation, and