Binding of diltiazem and verapamil to isolated rat heart mitochondria
โ Scribed by J. Rafael; J. Patzelt
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 378 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0300-8428
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๐ SIMILAR VOLUMES
An improved procedure for the isolation of mitochondria in high yields from normal and oxygen-deficient myocardium is described. The heart muscle is digested with Nagarse and homogenized simultaneously using a Polytron tissue homogenizer. Mitochondria are isolated by differential centrifugation, and
Bioenergetics of isolated lung and heart mitochondria from adult and aged rats were examined in the presence of glutamate (NAD-linked substrate) or succinate + rotenone (FAD-linked substrate) following ozone exposure (3.0 ppm, 8 hr). In controls, several differences were observed between adults and