Isolation and characterization of maltose non utilizing (mnu) mutants mapping outside the MAL1 locus in Saccharomyces cerevisiae
β Scribed by Marco Vanoni; Michael J. Goldenthal
- Book ID
- 109316792
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 416 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0378-1097
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π SIMILAR VOLUMES
The MAL1 locus of Saccharomyces cerevisiae comprises three genes necessary for maltose utilization: a regulatory (MALR), a maltose transport (MALT) and a maltase gene (MALS). A fine structure genetic map of the MAL1R gene was constructed and the order of mutations was confirmed by plasmid-mediated c
The cloned DNA polymerase I gene has been used to map the POL1 locus on the left arm of chromosome XIV, between MET4 and TOP2. Temperature-sensitive mutants in POL1 have been obtained by in vitro mutagenesis of the cloned gene and in vivo replacement of the wild-type allele with the mutated copy. Ph