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Isolation and characterization of inhibitors of animal cell-free protein synthesis from barley seeds

โœ Scribed by Katsuhiko Asano; Birte Svensson; Flemming M. Poulsen


Publisher
Springer-Verlag
Year
1984
Weight
724 KB
Volume
49
Category
Article
ISSN
0105-1938

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Proteins present in messenger ribonucleoprotein particles were labeled with [35S]-methionine in Ehrlich ascites tumor cells in which synthesis of new ribosomes was inhibited. Poly(A)-protein complexes were isolated from free and membrane-bound polyribosomes by sucrose gradient centrifugation and aff