Isolation and characterization of brain lipids by solid phase extraction and thin layer chromatography
β Scribed by DC Schweisguth; FW Quelle; G Wachob; RH Hammerstedt
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 372 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0307-4412
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as starting material generally moved to the solvent front, while the unsaturated alcohols, being more polar, had lower RI;I values. Apart from providing useful TLC separation procedures, these results also demonstrate that addition of mercaptoacetic acid to nonoene fatty acids, esters and alcohols
Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and deter