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Isolation and Characterization of an Oxygen Sensitive Mutant ofAzorhizobium caulinodans

โœ Scribed by A. Das, M. L. Lodha

Book ID
118774882
Publisher
Springer-Verlag
Year
1997
Tongue
English
Weight
655 KB
Volume
6
Category
Article
ISSN
0971-7811

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Hydrogenase-negative (Hup-) mutants of Azorhizobium caulinodans ORS571 were isolated by means of Tn5 mutagenesis. The colony test used for screening for Hupstrains was based on the absence of reduction of triphenyltetrazolium chloride with hydrogen. Suspensions from cultures of the mutant strains gr