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Isolation and characterization of an aluminum-sensitive mutant of Pseudomonas fluorescens

✍ Scribed by Vasu D Appanna; Michael Rauh; Robert Hamel; Eric Gauthier


Book ID
109321747
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
466 KB
Volume
152
Category
Article
ISSN
0378-1097

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## Abstract The extracellular endopeptidase synthesized by soil bacterium __Pseudomonas fluorescens__ was purified to homogeneity in a four‐step procedure. The enzyme was purified 45‐fold, with a 20% recovery. The endopeptidase appeared to be a monomer with a molecular mass of approx. 50 kDa. The e