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Isolation and characterization of an hydroxykynureninase from homogenates of adult mouse liver

โœ Scribed by C.E. McDermott; D.A. Casciano; F.H. Gaertner


Book ID
118854560
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
317 KB
Volume
51
Category
Article
ISSN
0006-291X

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A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 X 10(7) cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characte