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Isolation and characterization of an auxin-inducible glutathioneS-transferase gene ofArabidopsis thaliana

✍ Scribed by Dianne A. M. Kop; Monique Schuyer; Ben Scheres; Bert J. Zaal; Paul J. J. Hooykaas

Publisher: Springer
Year: 1996
Tongue: English
Weight: 730 KB
Volume: 30
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00019016

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✦ Synopsis

Genes homologous to the auxin-inducible Ntl03 glutathione S-transferase (GST) gene of tobacco, were isolated from a genomic library of Arabidopsis thaliana. We isolated a 2 clone containing an auxininducible gene, AtlO3-1a, and part of a constitutively expressed gene, AtlO3-1b. The coding regions of the Arabidopsis genes were highly homologous to each other and to the coding region of the tobacco gene but distinct from the GST genes that have been isolated from arabidopsis thusfar. Overexpression of a cDNA clone in Escherichia coli revealed that the AT103-1A protein had GST activity.

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