Isolation and characterization of two cDNA clones that are rapidly induced during the wound response ofArabidopsis thaliana
✍ Scribed by Cheol-Soo Kim; June-Myoung Kwak; Hong-Gil Nam; Ki-Chung Kim; Baik-Ho Cho
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 529 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0721-7714
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✦ Synopsis
Differential screening of a cDNA library of Arabidopsis thaliana constructed from the plant tissues harvested 1 h after wounding resulted in isolation of 2 wound-inducible cDNA clones. Kinetic analysis revealed that the corresponding genes are rapidly induced upon wounding. Expression of these clones reached the maximum level around 1-1.5 h after wounding and then were progressively reduced. The time by which expression returned to the control level was around 3 h after wounding. Partial sequence analysis revealed that the two clones are highly homologous to the S-adenosylmethionine synthetase and the glutathione-S-transferase gene, respectively.