✦ LIBER ✦

Isolation and characterization of a cloned growth factor dependent macrophage cell line, BAC1.2F5

✍ Scribed by Claudia Morgan; Jeffrey W. Pollard; E. Richard Stanley

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 988 KB
Volume: 130
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041300316

No coin nor oath required. For personal study only.

✦ Synopsis

The SV40 transformed murine macrophage cell line, BACl, proliferates in response to the colony stimulating factor, CSF-1 (Schwarzbaum et al., J. Immunol., 7321158, 1984). In order to obtain a cell line suitable for biochemical and genetic studies of CSF-1 signal transduction, clones of BACl were established. Clones ranged from being completely autonomous to being completely dependent on CSF-1 for growth. Cells of one clone (2F5), which proliferated in response to either CSF-1 or granulocyte-macrophage CSF (GM-CSF) were characterized in detail. The kinetics of receptor-mediated internalization and intracellular destruction of CSF-1 were comparable to the kinetics observed with peritoneal exudate macrophages. CSF-1 was shown to regulate cell spreading, cell survival, protein degradation, and the duration of the GI and S phases of the cell cycle. The 2F5 clone therefore exhibits a number of CSF-1 stimulated responses and is being used for genetic and biochemical studies of CSF-1 action.

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