Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen

✍ Scribed by J. Dolfing; J. Zeyer; P. Binder-Eicher; R. P. Schwarzenbach

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 560 KB
• Volume: 154
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00276528

✦ Synopsis

A bacterium tentatively identified as a Pseudomonas sp. was isolated from a laboratory aquifer column in which toluene was degraded under denitrifying conditions. The organism mineralized toluene in pure culture in the absence of molecular oxygen. In carbon balance studies using [ring-UL-14C]toluene, more than 50% of the radioactivity was recovered as 14CO2. Nitrate and nitrous oxide served as electron acceptors for toluene mineralization. The organism was also able to degrade m-xylene, benzoate, benzaldehyde, p-cresol, p-hydroxy-benzaldehyde, p-hydroxybenzoate and cyclohexanecarboxylic acid in the absence of molecular oxygen.