Isolation and characterization of 5-lipoxygenase from tulip bulbs

✍ Scribed by P. Reddanna; J. Whelan; P.S. Reddy; C.C. Reddy

• Book ID: 117058469
• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 317 KB
• Volume: 157
• Category: Article
• ISSN: 0006-291X
• DOI: 10.1016/s0006-291x(88)81023-4

✦ Synopsis

An unique membrane bound lipoxygenase was isolated and purified from purple star tulip bulbs with a specific activity of 5.2 mu moles O2 consumed.min-1.mg-1 protein. The purified tulip enzyme exhibits regiospecificity for O2 insertion at C-5 of the arachidonic acid molecule. Identification of the reaction product was confirmed as 5-hydroperoxyeicosatetraenoic acid by analytical criteria which included: cochromatography with the authentic compound, as well as mass spectral and 1H-NMR analysis. Thus, the enzyme from tulip bulbs appears to be different from the cytosolic lipoxygenase from potato tubers, which exhibits non-regiospecificity in terms of O2 incorporation. However, the purified tulip lipoxygenase showed a strong immunological crossreactivity with antiserum raised against the purified potato lipoxygenase, indicating close immunological relationship with the other plant lipoxygenases.