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Isoelectric points and conformation of proteins: I. Effect of urea on the behavior of some proteins in isoelectric focusing

โœ Scribed by Nobuo Ul


Book ID
115749004
Publisher
Elsevier Science
Year
1971
Weight
884 KB
Volume
229
Category
Article
ISSN
0005-2795

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Effect of urea on pI's of ampholytes and
โœ R.V. Josephson; S.K. Maheswaran; C.V. Morr; R. Jenness; R.K. Lindorfer ๐Ÿ“‚ Article ๐Ÿ“… 1971 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 436 KB

Urea has been used to dissociate and solubilize proteins for isoelectric focusing (electrofocusing) in acrylamide gels (llJ8) and sucrose gradient columns (2,3,4,17). In using concentrated urea (7 M) in electrofocusing experiments with whole bovine casein, we observed that the pH of the ampholyte gr