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Effects of ampholyte concentration on protein behavior in on-chip isoelectric focusing

โœ Scribed by Jaesool Shim; Prashanta Dutta; Cornelius F. Ivory

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
627 KB
Volume
29
Category
Article
ISSN
0173-0835

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โœ R.V. Josephson; S.K. Maheswaran; C.V. Morr; R. Jenness; R.K. Lindorfer ๐Ÿ“‚ Article ๐Ÿ“… 1971 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 436 KB

Urea has been used to dissociate and solubilize proteins for isoelectric focusing (electrofocusing) in acrylamide gels (llJ8) and sucrose gradient columns (2,3,4,17). In using concentrated urea (7 M) in electrofocusing experiments with whole bovine casein, we observed that the pH of the ampholyte gr