IS2-43 and IS2-44: New alleles of the insertion sequence IS2 which have promoter activity

✍ Scribed by Sommer, Hans ;Cullum, John ;Saedler, Heinz

• Book ID: 104693244
• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 487 KB
• Volume: 175
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00267855

✦ Synopsis

The sequence of two new IS2 alleles with promoter activity (IS2-43 and IS2-44) is reported. The alleles are identical and are formed by a 17 bp tandem duplication in an AT-rich region of IS2. This created a new RNA polymerase binding site. A mutation was found that increased the frequency of formation of these 17 bp duplications but not of another class of duplications, the "mini-insertions". This suggested that the mechanisms of formation of the two classes of duplications are different.