Is the histochemical demonstration of lipase activity possible?
β Scribed by Lake, Brian D.
- Publisher
- Springer
- Year
- 1972
- Tongue
- English
- Weight
- 227 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0018-2214
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Difficulties in the histochemical demonstration of even maximum G6Pase activity can be overcome when unfixed cryostat sections are immersed in a medium which is shaken during incubation. The medium contains: 20mM G6P, 4.6 mM Pb(NO3)2, 50 mM tris-maleate buffer, pH 6.5.
The "direct coloring" thiocholine method of Karnovsky and Roots (1964) for the demonstration of acetylcholinesterase (ACHE) activity was modified and adapted to the technique of semipermeable membranes. In this way it is possible to demonstrate histochemically both the bound as well as the soluble p