✦ LIBER ✦

A technical note on the histochemical demonstration of G6Pase activity

✍ Scribed by I. P. Maly; D. Sasse

Publisher: Springer
Year: 1983
Tongue: English
Weight: 491 KB
Volume: 78
Category: Article
ISSN: 1432-119X
DOI: 10.1007/bf00496628

No coin nor oath required. For personal study only.

✦ Synopsis

Difficulties in the histochemical demonstration of even maximum G6Pase activity can be overcome when unfixed cryostat sections are immersed in a medium which is shaken during incubation. The medium contains: 20mM G6P, 4.6 mM Pb(NO3)2, 50 mM tris-maleate buffer, pH 6.5.

📜 SIMILAR VOLUMES

Quantitative determination of G6Pase act

Quantitative determination of G6Pase activity in histochemically defined zones of the liver acinus

✍ H. F. Teutsch 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 976 KB

Qualitative histochemical G6Pase distribution patterns obtained with an improved method (Teutsch, 1978) served as the basis for a zonal microdissection of the liver acinus. G6Pase activity was determined quantitatively in tissue samples of zones 1 and 3 by a microfluorometric method (Burch et al., 1

Histochemical demonstration of G6PDH- an

Histochemical demonstration of G6PDH- and SDH-activity on cultured fibroblastoid cells from rat pancreas using a “membrane technique”

✍ G. Weiß; G. Vergani; S. Pentz 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 376 KB

The applied "membrane technique" represents a simple and time-saving method to provide evidence for presence of enzyme activities in single cultured cells. Substrates for G6PDH and SDH are filled on a semipermeable membrane stretched over a cylinder of plastic. The cells are cultured on a polyester

Histochemical technique for the demonstr

Histochemical technique for the demonstration of pyruvate kinase activity

✍ A. E. F. H. Meijer; R. Hoven 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 786 KB

A histochemical method for the demonstra

A histochemical method for the demonstration of acetylcholinesterase activity using semipermeable membranes

✍ J. Andrä; Z. Lojda 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 953 KB

The "direct coloring" thiocholine method of Karnovsky and Roots (1964) for the demonstration of acetylcholinesterase (ACHE) activity was modified and adapted to the technique of semipermeable membranes. In this way it is possible to demonstrate histochemically both the bound as well as the soluble p

Histochemical demonstration of a glycoca

Histochemical demonstration of a glycocalyx on the cell surface ofHeterosigma akashiwo

✍ M. Yokote; T. Honjo; M. Asakawa 📂 Article 📅 1985 🏛 Springer-Verlag 🌐 English ⚖ 845 KB

Biochemical investigation to the reliabi

Biochemical investigation to the reliability of the histochemical demonstration of microsomal arylsulphatase activity in cryostat sections

✍ C. M. Loos; A. J. Breda; A. E. F. H. Meijer; A. C. Jöbsis 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 264 KB

Polyacrylamide gel-electrophoresis was performed with an extract from cultivated skin fibroblasts. Arylsulphatase activity is measured and visualised using the biochemical substrate dehydroepiandrosterone sulphate and the histochemical substrate 6-bromo-2-naphthyl sulphate respectively. The histoche