✦ LIBER ✦

Irreversible binding of14C-labelled trichloroethylene to mice liver constituents in vivo and in vitro

✍ Scribed by H. Uehleke; Sonja Poplawski-Tabarelli

Publisher: Springer-Verlag
Year: 1977
Tongue: English
Weight: 381 KB
Volume: 37
Category: Article
ISSN: 0340-5761
DOI: 10.1007/bf00330820

No coin nor oath required. For personal study only.

✦ Synopsis

14C-labelled trichloroethylene was injected i.p. into male mice (10 mumole/g of b.w.). The radioactivity irreversibly bound to hepatic protein reached highest levels after 6 h: 2 nmole/mg in cytosol protein, 4.4 nmole/mg in mitochondrial protein, and 7.6 nmole/mg in microsomal protein. 2. The commercial trichloroethylene contained radioactive impurities binding to proteins without metabolic activation. Purification by various extractions removed 60-70% of those materials. In aerobic incubates of mice hepatic microsomes and NADPH the covalent binding rate of the purified trichloroethylene was 1.4 nmole/mg protein in 60 min. The activity of rat liver microsomes was approximately 40% less. Covalent binding increased 2-fold with microsomes of mice pretreated with phenobarbital.

📜 SIMILAR VOLUMES

In vitro binding of 14c-misonidazole to

In vitro binding of 14c-misonidazole to hepatocytes and hepatoma cells debora

✍ Debora J. Van Os-corby; J. Donald Chapman 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 427 KB

The outstanding metabolic stability of a

The outstanding metabolic stability of a 14C-labeled β-nonapeptide in rats – in vitro and in vivo pharmacokinetic studies

✍ Hansjörg Wiegand; Bernard Wirz; Alain Schweitzer; Gian P. Camenisch; Maria I. Ro 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB 👁 1 views

The covalent binding of cyclosporin a to

The covalent binding of cyclosporin a to rat liver macromolecules in vivo and in vitro: The role of cytochrome P-450

✍ J. Fred Nagelkerke; Roeline B. Tijidens; Erik P. Schwarz; Marjolein F.G. Winters 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 383 KB

Effects of vitamin A on the metabolism a

Effects of vitamin A on the metabolism and mutagenicity of 2AAF in vitro and on the covalent binding to rat liver DNA/RNA in vivo

✍ Lars Rondahl; Leif Busk; Ulf G. Ahlborg; Kerstin Bergman 📂 Article 📅 1985 🏛 Springer-Verlag 🌐 English ⚖ 518 KB

Down-regulation of particulate protein k

Down-regulation of particulate protein kinase Cϵ and up-regulation of nuclear activator protein–1 DNA binding in liver following in vivo exposure of B6C3F1 male mice to heptachlor epoxide

✍ Mark E. Hansen; Fumio Matsumura 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 275 KB 👁 1 views

Role of mixed-function oxidases and non-

Role of mixed-function oxidases and non-protein sulfhydryl contentent in [14C]-2-chloro-4-acetotoluidide binding to liver and kidney in starlings

✍ Shri N. Giri; David M. Siegel 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 924 KB

The compound 2-chloro-4-acetotoluididc (CAT) is highly toxic to many avian species, including the starling. In our earlier work, we demonstrated the covalent binding of radioactivity from [14C]-CAT to liver and kidneys of the starling. In the present study, the effects of inducers of mixed-function