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Ion/ion chemistry as a top-down approach for protein analysis

✍ Scribed by James L Stephenson Jr; Scott A McLuckey; Gavin E Reid; J.Mitchell Wells; Jonathan L Bundy

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
467 KB
Volume
13
Category
Article
ISSN
0958-1669

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✦ Synopsis

Developing methodology for analyzing complex protein mixtures in a rapid fashion is one of the most challenging problems facing analytical biochemists today. Recent advances in mass spectrometry for the analysis of intact proteins (i.e. the top-down approach) show great promise for rapid protein identification. The ion/ion chemistry approach for the detection and identification of target proteins in complex matrices, determination of fragmentation channels as a function of precursor ion charge state, and post-translational modification characterization are discussed with particular emphasis on tandem mass spectrometry of intact proteins.

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