Ionic liquid-based aqueous two-phase extraction of selected proteins
β Scribed by Yuanchao Pei; Jianji Wang; Kun Wu; Xiaopeng Xuan; Xinjie Lu
- Book ID
- 108262409
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 559 KB
- Volume
- 64
- Category
- Article
- ISSN
- 1383-5866
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## Abstract The ionic liquid (IL) Ammoeng110β’ contains cations with oligoethyleneglycol units and was found to be highly effective for the formation of aqueous twoβphase systems (ATPS) that can be used for the biocompatible purification of active enzymes. Above critical concentrations of the IL and
An iminodiacetic acid derivative of poly(ethylene glycol) (PEG-IDA) that chelates metal cations has been synthesized and used to extract proteins in metal affinity aqueous two-phase PEG/ dextran systems. With less than 1% of the PEG substituted with chelated copper, partition coefficients are shown