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Ion pairing high-performance liquid chromatography of catecholamines

✍ Scribed by Heady, Judith E. ;Agee, Karl D.

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
259 KB
Volume
2
Category
Article
ISSN
0935-6304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Catecholamine standards have been separated by ion pairing reversed‐phase high‐performance liquid chromatography (HPLC). A trace substance was separable from four standards when low concentrations of methanol were used in the elution buffer. This method has allowed separation of an unknown uterine catecholamine, partially purified on a boronate affinity gel column, from the standards: norepinephrine, epinephrine, normetanephrine and metanephrine.

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