Ion-pair HPLC determination of hydroxycinnamic acid monoconjugates of putrescine, spermidine and spermine

✍ Scribed by Candace Panagabko; Deborah Chenier; Solomon Fixon-Owoo; Jeffrey K. Atkinson

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 104 KB
• Volume: 11
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(200001/02)11:1<11::aid-pca479>3.0.co;2-0

✦ Synopsis

The terminal hydroxycinnamic acid monoconjugates of putrescine, spermidine and spermine were resolved and quantified in extracts of plant tissues using sodium hexanesulphonate as an ion-pair reagent and a double methanol gradient. Extracts were prepared from acidic methanol:water (80:20) that had been backextracted with hexane, and fractionated on C-18 solid phase extraction and carboxymethyl cation exchange cartridges. The chromatographic conditions were capable of separating N 1 -and N 8 -coumaroyl and feruloyl spermidine, which differ only by the disposition of the secondary amine in the polyamine side chain. Reliable detection limits for most compounds were less than 5 mg/mL (approximately 10 À5 M in the plant extract) and extraction efficiencies were 91% as determined using the novel internal standard, N 1 -coumaroyl hexanediamine.