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High-speed liquid chromatographic determination of putrescine, spermidine and spermine

โœ Scribed by Teruyuki Sugiura; Tokishi Hayashi; Satoshi Kawai; Takeo Ohno

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
254 KB
Volume
110
Category
Article
ISSN
1873-3778

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The terminal hydroxycinnamic acid monoconjugates of putrescine, spermidine and spermine were resolved and quantified in extracts of plant tissues using sodium hexanesulphonate as an ion-pair reagent and a double methanol gradient. Extracts were prepared from acidic methanol:water (80:20) that had be