✦ LIBER ✦

Ion-pair assisted recovery of matrix-assisted laser desorption/ionization mass spectral signals from SDS-containing peptide–protein mixtures

✍ Scribed by Rajendram V. Rajnarayanan; Kuan Wang

Publisher: John Wiley and Sons
Year: 2004
Tongue: English
Weight: 538 KB
Volume: 39
Category: Article
ISSN: 1076-5174
DOI: 10.1002/jms.572

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✦ Synopsis

Abstract

We have developed a simple and effective means of using alkylammonium ion‐pairing agents, such as cetyltetramethylammonium bromide, to recover matrix assisted laser desorption/ionization mass spectrometric (MALDI‐MS) signals from sodium dodecyl sulfate (SDS)‐containing protein and peptide samples. A two‐layer method of matrix preparation, with a bottom matrix layer of ion‐pairing agents and a top matrix layer of SDS protein samples, is essential for reproducible MALDI mass spectra with good recovery. Both buffer ions and ion‐pairing agents have profound effects on signal recovery and can be rapidly and systematically optimized. This practical technique, termed ion‐pair assisted recovery (IPAR), is compatible with major SDS‐based biotechniques and can be easily incorporated into high‐throughput proteomic analysis. Copyright © 2004 John Wiley & Sons, Ltd.

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