## Abstract Effects of pH and conductivity on the ion exchange chromatographic purification of an antigenโbinding antibody fragment (Fab) of pI 8.0 were investigated. Normal sulfopropyl (SP) group modified agarose particles (SP Sepharoseโข Fast Flow) and dextran modified particles (SP Sepharose XL)
Ion-exchange chromatography of the streptomycin complex
โ Scribed by Pereira, J. N.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1961
- Weight
- 322 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0368-1467
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โฆ Synopsis
Abstract
Various studies have revealed the presence of several maltolproducing compounds in streptomycin preparations. These previous studies have utilized the techniques of counterโcurrent distribution, column chromatography, paper chromatography, and electrophoresis. A system of cationโexchange chromatography has been reported which separates, at least partially, six components of the streptomycin complex. This technique involves the use of a gradient elution procedure.
In addition to five maltolโproducing compounds, another member of the complex is reported. The possible significance of such a compound is discussed.
๐ SIMILAR VOLUMES
Luven (1) used column ion-exchange chromatography developed by Spackman, Stein, and Moore (2) for the estimation of tryptophan in alkaline hydrolyzates of crude plant proteins. The procedure was time consuming, and due to the analytical difficulties involved variable tryptophan values were obtained.