## Abstract Ionβexchange (IEX) chromatography steps are widely applied in protein purification processes because of their high capacity, selectivity, robust operation, and wellβunderstood principles. Optimization of IEX steps typically involves resin screening and selection of the pH and counterion
Ion Chromatography || Principles of Ion Chromatographic Separations
β Scribed by Fritz, James S.; Gjerde, Douglas T.
- Publisher
- Wiley-VCH Verlag GmbH & Co. KGaA
- Year
- 2009
- Tongue
- German
- Weight
- 180 KB
- Edition
- 4
- Category
- Article
- ISBN
- 3527320520
No coin nor oath required. For personal study only.
β¦ Synopsis
This separation method is increasingly finding applications in environmental, food, pharmaceutical and clinical analysis.
The completely revised and updated fourth edition of this best-selling classic is a thorough treatment of the subject while remaining concise and readable. Alongside ion exchange resins, detectors, ion chromatographic separations, anion, cation and ion exclusive chromatography, special techniques and chemical speciation, new additions include capillary electrophoresis, monolithic columns, zwitterion columns, DNA/RNA analysis, fundamentals of the science of IC, and micro methods.
The whole is rounded off by handy tables with details on, among others, detection or elution conditions, making this a ready reference for analytical, environmental and food chemists, chromatographers, pharmaceutists, and chemists working in trace analysis.
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Isocratic elution with imidazole of some protein models from a chelating gel, CSFF-IDA-M(II), resulted in their desorption owing to the low pH (644) of the mobile phase rather than to the imidazole itself (idazole-generated fall in pH; proton pump). Gradient elution with imidazole was best accomplis
## Abstract Effects of pH and conductivity on the ion exchange chromatographic purification of an antigenβbinding antibody fragment (Fab) of pI 8.0 were investigated. Normal sulfopropyl (SP) group modified agarose particles (SP Sepharoseβ’ Fast Flow) and dextran modified particles (SP Sepharose XL)
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