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Iodometric and spectrophotometric assays for cephradine after its hydrolysis with a β-lactamase

✍ Scribed by B. May Frantz

Book ID
102914302
Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
634 KB
Volume
65
Category
Article
ISSN
0022-3549

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✍ R.F. Pratt; W.Stephen Faraci; Chandrika P. Govardhan 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 258 KB

A direct spectrophotometric pH indicator method has been devised to assay the activity of the D-Ala carboxypeptidase/transpeptidase of Streptomyces R61, and which should be of general application to D-Ala carboxypeptidases. The substrate employed is N,N'diacetyl-r.lysyl-D-alanyl-D-lactate. The metho