𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Involvement of TRAIL/TRAIL-receptors in human intestinal cell differentiation

✍ Scribed by Erika Rimondi; Paola Secchiero; Andrea Quaroni; Carlotta Zerbinati; Silvano Capitani; Giorgio Zauli

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
328 KB
Volume
206
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Despite the fact that tumor necrosis factor (TNF)‐related apoptosis inducing ligand (TRAIL) and its receptors (TRAIL‐Rs) are expressed in intestinal mucosa, little is known about the biological role of this system in intestinal cell physiology. The expression of surface TRAIL and TRAIL‐R1, ‐R2, ‐R3, ‐R4 were examined by flow cytometry in the immortalized human cell line tsFHI under culture conditions promoting growth or growth arrest and expression of differentiated traits. A progressive increase of surface TRAIL expression paralleled tsFHI differentiation, consistently with immunohistochemistry analysis showing an increase of TRAIL immunostaining along the crypt–villus axis in normal jejuneal mucosa. In spite of the presence of TRAIL‐R1 and TRAIL‐R2 “death receptors,” recombinant TRAIL was not cytotoxic for tsFHI cells. Exposure of tsFHI to recombinant TRAIL rather increased/anticipated the expression levels of the cyclin‐dependent kinase inhibitors p21 and p27, which mediate the induction of growth arrest and the stabilization of differentiated traits, respectively, as well as of the canonical differentiation marker DPPIV. The differentiation inducing activity of TRAIL was abolished by pre‐incubation with a Fc‐TRAIL‐R2 chimera. On the other hand, TRAIL did not significantly modulate the levels of osteoprotegerin (OPG), CXCL8/IL‐8, CXCL9/MIG, and CXCL10/IP10 spontaneously released or induced by inflammatory cytokines. Taken together, these data suggest that TRAIL might act as a paracrine trophic cytokine on intestinal epithelium, promoting intestinal cell differentiation. J.Cell.Physiol. © 2005 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Mutation analysis and mRNA expression of
Mutation analysis and mRNA expression of trail-receptors in human breast cancer
✍ Susanne Seitz; Peter Wassmuth; Jörg Fischer; Anita Nothnagel; Burkhard Jandrig; 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 316 KB 👁 2 views

## Abstract The chromosome region 8p12‐p22 shows frequent allelic loss in a variety of human malignancies, including breast cancer (BC). The tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL)‐receptors TRAIL‐R1, ‐R2, ‐R3 and ‐R4 are located on 8p21‐p22 and might be candidate tumor supp

IFN-γinduces cell death in human hepatom
IFN-γinduces cell death in human hepatoma cells through a trail/death receptor-mediated apoptotic pathway
✍ Eui-Cheol Shin; Ju Mi Ahn; Chul Hoon Kim; Youjeong Choi; Young Soo Ahn; Hoguen K 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 281 KB

We demonstrated the induction of cell death in a hepatoma cell line by IFN-gamma and its possible mechanism. Among the 2 hepatitis B virus (HBV)-associated hepatoma cell lines, SNU-354 and SNU-368, IFN-gamma induced cell death and increased caspase-3 activity in SNU-368 but not in SNU-354. IFN-gamma

TRAIL receptor upregulation and the impl
TRAIL receptor upregulation and the implication of KRAS/BRAF mutations in human colon cancer tumors
✍ Eftychia Oikonomou; Vivian Kosmidou; Anastasia Katseli; Konstantinos Kothonidis; 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 French ⚖ 273 KB 👁 2 views

## Abstract TRAIL raises hopes as a promising anti‐tumor agent due to its selectivity toward cancer cells. Higher expression of its pro‐death receptors TRAIL‐R1 (DR4) and TRAIL‐R2 (DR5) attenuates higher sensitivity to TRAIL‐induced apoptosis, and represents a marker for better cancer prognosis and

α-TEA induces apoptosis of human breast
α-TEA induces apoptosis of human breast cancer cells via activation of TRAIL/DR5 death receptor pathway
✍ Weiping Yu; Richa Tiwary; Jing Li; Sook-Kyung Park; Li Jia; Ailian Xiong; Marla 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 343 KB

## Abstract Vitamin E derivative RRR‐α‐tocopherol ether‐linked acetic acid analog (α‐TEA) induces apoptosis in MCF‐7 and HCC‐1954 human breast cancer cells in a dose‐ and time‐dependent manner. α‐TEA induces increased levels of tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) and dea

Effect of hyperthermia and chemotherapeu
Effect of hyperthermia and chemotherapeutic agents on TRAIL-induced cell death in human colon cancer cells
✍ Jinsang Yoo; Yong J. Lee 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 712 KB

## Abstract Tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) is a promising cancer therapeutic agent because of its tumor selectivity. TRAIL is known to induce apoptosis in cancer cells but spare most normal cells. In the previous study [Yoo and Lee, 2007], we have reported that hype