Involvement of the Escherichia coli folate-binding protein YgfZ in RNA modification and regulation of chromosomal replication initiation
✍ Scribed by Tomotake Ote; Masayuki Hashimoto; Yoshiho Ikeuchi; Masayuki Su’etsugu; Tsutomu Suzuki; Tsutomu Katayama; Jun-Ichi Kato
- Book ID
- 110832870
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 32 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0950-382X
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Increased synthesis of DnaA protein, obtained with plasmids carrying the dnaA gene controlled by the heat inducible lambda pL promoter, stimulated initiation of replication from oriC about threefold. The overinitiation was determined both as an increase in copy number of a minichromosome and as an i
## Abstract **Background:** During the cell cycle, the initiation of chromosomal replication is strictly controlled. In __Escherichia coli__, the initiator DnaA and the replication origin __oriC__ are major targets for this regulation. Here, we assessed the role of transcription of the __mioC__ gen