Investigation of the applicability of a sequential digestion protocol using trypsin and leucine aminopeptidase M for protein identification by matrix-assisted laser desorption/ionization – time of flight mass spectrometry
✍ Scribed by Alan Doucette; Liang Li
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 162 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1615-9853
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Cation adduction to oligonucleotides and nucleic acids during mass spectrometric analysis is a recurrent problem. We have found that the use of organic base solutions of high gas-phase proton affinity, as used previously in electrospray ionization mass spectrometry, can significantly reduce the cati
RNase H is an endonuclease which cleaves RNA at points of hybridization with DNA. However, certain ambiguities exist in terms of its speciÐcity and location of cleavage along the RNA strand. The analysis of RNase H reaction products of an oligoribonucleotide hairpin by matrix-assisted laser desorpti