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Investigation of protein refolding using a fractional factorial screen: A study of reagent effects and interactions

✍ Scribed by Melissa Swope Willis; James K. Hogan; Prakash Prabhakar; Xun Liu; Kuenhi Tsai; Yunyi Wei; Ted Fox

Book ID
111752488
Publisher
Cold Spring Harbor Laboratory Press
Year
2005
Tongue
English
Weight
389 KB
Volume
14
Category
Article
ISSN
0961-8368

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Fluorescamine is a useful reagent in monitoring protein-DNA interactions only if a convenient method of separating the complex from free protein is available. Sedimentation of the complex provides such a method at least in the case of histone-like proteins capable of extensive interaction with DNA.