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Investigation of intracellular phosphate pools of the green alga Chlorella using 31P nuclear magnetic resonance

โœ Scribed by J. Sianoudis; A. Mayer; D. Leibfritz


Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
358 KB
Volume
22
Category
Article
ISSN
0749-1581

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โœฆ Synopsis


P NMR measurements were performed in order to study the intracellular phosphate pools of the green alga ChZoreZZu fusca. Cells of asynchronous cultures contain a large pool of polyphosphates, whereas the amount of polyphosphates in cells of synchronous cultures depends on the state of diflerentiation. The assignment of extracellular and intracellular inorganic orthophosphate is achieved by changing the pH, whereas the intracellular compartmentation of inorganic orthophosphates and sugar phosphates is shown during irradiation of the cells with light within the NMR spectrometer.


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