Investigation of intracellular phosphate pools of the green alga Chlorella using 31P nuclear magnetic resonance
โ Scribed by J. Sianoudis; A. Mayer; D. Leibfritz
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 358 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0749-1581
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โฆ Synopsis
P NMR measurements were performed in order to study the intracellular phosphate pools of the green alga ChZoreZZu fusca. Cells of asynchronous cultures contain a large pool of polyphosphates, whereas the amount of polyphosphates in cells of synchronous cultures depends on the state of diflerentiation. The assignment of extracellular and intracellular inorganic orthophosphate is achieved by changing the pH, whereas the intracellular compartmentation of inorganic orthophosphates and sugar phosphates is shown during irradiation of the cells with light within the NMR spectrometer.
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