In vivo evaluation of intracellular pH and high-energy phosphate metabolities during regional myocardial ischemia in cats using 31P nuclear magnetic resonance
✍ Scribed by Paul D. Stein; Sidney Goldstein; Hani N. Sabbah; Zhi-Quan Liu; Joseph A. Helpern; James R. Ewing; Jeffrey B. Lakier; Michael Chopp; William F. Lapenna; Kenneth M. A. Welch
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 451 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0740-3194
No coin nor oath required. For personal study only.
✦ Synopsis
Phosphorus-3 1 nuclear magnetic resonance spectroscopy (3'P NMR) was used to assess the temporal changes of high-energy phosphate metabolites in the region of acute myocardial ischemia of open-chest cats. Eight anesthetized cats were studied following ligation of the left anterior descending coronary artery. Creatine phosphate showed a 79 ? 16% (mean 2 SD) reduction by 4 min after the onset of ischemia. Prominent qualitative reductions of the spectral peak of creatine phosphate occurred by 40 s after ischemia. Adenosine triphosphate measured under the beta spectral peak (0-ATP) decreased 37 k 9% by 20-25 min after ligation of the left anterior descending coronary artery. These reductions developed more slowly and were of smaller magnitude than those of creatine phosphate. Intracellular pH decreased from 7.39 f 0.07 to 7.13 rfr 0.09 units by 40 s after ischemia. By 30 min, pH decreased to 6.07 ? 0.40 units. The study shows, therefore, the temporal changes of high-energy phosphate metabolites during ischemia in localized regions of the myocardium of open-chest animals.