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Investigating the Role of the Latch in the Positive Supercoiling Mechanism of Reverse Gyrase †

✍ Scribed by Rodríguez, A. Chapin

Book ID
127168251
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
346 KB
Volume
42
Category
Article
ISSN
0006-2960

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This study deals with the effects of a temperature-sensitive (ts) mutation at the gene encoding the DNA gyrase B subunit (gyrBts) and a deletion of the top gene encoding the omega protein upon the superhelical density of the pAO3 plasmid in E. coli cells. The alteration of the DNA gyrase B subunit i