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The DNA-Gate of Gyrase Is Predominantly in the Closed Conformation During DNA Supercoiling

✍ Scribed by Gubaev, Airat; Hilbert, Manuel; Klostermeier, Dagmar

Book ID
119206411
Publisher
Biophysical Society
Year
2010
Tongue
English
Weight
74 KB
Volume
98
Category
Article
ISSN
0006-3495

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This study deals with the effects of a temperature-sensitive (ts) mutation at the gene encoding the DNA gyrase B subunit (gyrBts) and a deletion of the top gene encoding the omega protein upon the superhelical density of the pAO3 plasmid in E. coli cells. The alteration of the DNA gyrase B subunit i