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Introduction to the biosafety guidelines for sorting of unfixed cells

✍ Scribed by Ingrid Schmid; Phillip N. Dean

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 16 KB
Volume: 28
Category: Article
ISSN: 0196-4763
DOI: 10.1002/(sici)1097-0320(19970601)28:2<97::aid-cyto1>3.0.co;2-d

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✦ Synopsis

Cell sorting of biologic specimens by flow cytometry has become a widely used technique in research and clinical laboratories. Because viable biologic specimens can contain infectious agents, precautions need to be taken to prevent the exposure of operators of flow cytometers to biohazards arising from the use of these instruments. The International Society of Analytical Cytology (ISAC) recognized the need for establishing guidelines for sorting of unfixed cells and initiated the formation of a Biohazard Working Group, which has produced a set of recom-mendations that can aid in preventing such exposures. The guidelines contain recommendations on 1) sample handling, 2) training and protection of operators, 3) laboratory design, 4) cell sorter setup and maintenance, and 5) testing the instrument for the efficiency of aerosol containment. Cytometry 28

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